FLUO Series

Epi Fluorescence microscopes
A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study properties of organic or inorganic substances. The “fluorescence microscope” refers to any microscope that uses fluorescence to generate an image. The Epi Fluorescence microscope is equipped with a fluorescence illuminator wich generates incident fluorescence light.highlight points of interest.
Principle
The specimen is illuminated with light of a specific wavelength (or wavelengths) which is absorbed by the fluorophores, causing them to emit light of longer wavelengths (i.e., of a different color than the absorbed light). The illumination light is separated from the much weaker emitted fluorescence through the use of a spectral emission filter. Typical components of a fluorescence microscope are a light source (HBO mercury-vapor lamps are common; more advanced forms are high-power LEDs), the excitation filter, the dichroic mirror, and the emission filter. The filters and the dichroic mirror are chosen to match the spectral excitation and emission characteristics of the fluorophore used to label the specimen. In this manner, the distribution of a single fluorophore (color) is imaged at a time. Multi-color images of several types of fluorophores must be composed by combining several single-color images. Most fluorescence microscopes in use are epifluorescence microscopes, where excitation of the fluorophore and detection of the fluorescence are done through the same light path (through the objective). These microscopes are widely used in biology and are the basis for more advanced microscope designs.
Epifluorescence microscopy
The majority of fluorescence microscopes, especially those used in the life sciences, are of the epifluorescence design. Light of the excitation wavelength illuminates the specimen through the objective lens. The fluorescence emitted by the specimen is focused to the detector by the same objective that is used for the excitation which for greater resolution will need objective lens with higher numerical aperture. Since most of the excitation light is transmitted through the specimen, only reflected excitatory light reaches the objective together with the emitted light and the epifluorescence method therefore gives a high signal-to-noise ratio. The dichroic beamsplitter acts as a wavelength specific filter, transmitting fluoresced light through to the eyepiece or detector, but reflecting any remaining excitation light back towards the source.

 

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FLUORESCENCE

The fluorescence microscopy is the most demanding technique in biology and the biomedical sciences, as well as in materials science.
This method is capable to study organic and inorganic samples thanks to primary fluorescence (auto-fluorescence) or secondary (staining and labelling with fluorochromes).

Fluorescence_Paper_4x_B-383LD2_Blu.33e

VALUABLE SUPPORT TO MALARIA & TBC DIAGNOSIS

The B-383LD1 LED fluorescent microscope is a cutting-edge solution designed to enable rapid diagnosis of malaria and TBC, by using acridine-orange staining technique. The optional no cover glass W-PLAN 50x/0.75 objective is required to ensure excellent results for stunning images.

The aim is to reduce the costs to a minimum and provide a cost-effective, intuitive but performing solution, purposely designed for these applications.

Multiple Observation Methods Available

IM-5FLD means extreme flexibility.
In fact, brightfield, phase contrast, darkfield and fluorescence techniques can be used at any time, to highlight different aspects of your specimen.

Here you can see a Rhizopus sample, seen under (from top to bottom): Blue Excitation, Green Excitation, Ultraviolet Excitation, combination of Green Excitation and Phase Contrast, Phase Contrast

MOTORIZED LED SOURCE SELECTION

Using LED fluorescence has never been so easy!
With IM-5FLD, you will simply choose which filter is needed (among blue, green and UV) and then the microscope will select the most suitable lighting source for you, automatically.

In addition, an extra slot is available for the most demanding users, with the possibility to create a customized configuration.

Images are for illustrative purposes only

B-290LD - LED Fluorescence Microscopes

B-292LD1.50_models

Model:  B-292LD1.50


Fluorescence binocular microscopes especially designed for tubercolosis and malaria analysis with IOS W-PLAN MET 50x/0.75 (No Cover), with anti-fungus treatment.

B-293LD1.50_models

Model:  B-293LD1.50


Fluorescence trinocular microscopes especially designed for tubercolosis and malaria analysis with IOS W-PLAN MET 50x/0.75 (No Cover), with anti-fungus treatment.

B-292LD1_models

Model:  B-292LD1


Fluorescence binocular microscopes especially designed for tubercolosis and malaria analysis IOS W-PLAN 100x/0.80 (No Cover, Dry), with anti-fungus treatment.

B-293LD1_models

Model:  B-293LD1


Fluorescence trinocular microscopes especially designed for tubercolosis and malaria analysis with IOS W-PLAN MET 50x/0.75 (No Cover), with anti-fungus treatment.

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B-383LD - LED Fluorescence Microscope

B-383LD1_model

Model:  B-383LD1


Entry-level laboratory upright microscope for brightfield and fluorescence observations with IOS N-PLAN objectives. The extremely powerful LED fluorescence illuminator is combined with blue excitation filter set for the visualization of the following fluorochromes: Acridine Yellow, Acridine Orange, Auramine, DiO, DTAF, FITC, GFP, YFP, etc. LED fluorescence ensures unparalleled convenience eliminating warm-up/cool-down times and all the inconveniences related lamp replacement and adjustment. Transmitted light through the exclusive X-LED3 to ensure great-looking, rich and high-quality specimen view.

B-383LD1_model

Model:  B-383LD2


Laboratory upright microscope for brightfield and fluorescence observations with IOS N-PLAN objectives. The extremely powerful LED fluorescence illuminator is combined with blue and green excitation filter set for the visualization of the following fluorochromes: Acridine Yellow, Acridine Orange, Auramine, DiO, DTAF, FITC, GFP, YFP, etc. (blue) plus Rhodamine, Texas Red and TRITC (green). LED fluorescence ensures unparalleled convenience eliminating warm-up/cool-down times and all the inconveniences related lamp replacement and adjustment. Transmitted light through the exclusive X-LED3 to ensure great-looking, rich and high-quality specimen view.

B-383FL - HBO Fluorescence Microscope

B-383LD1_model

Model:  B-383FL


Laboratory upright microscope for brightfield and fluorescence observations with IOS N-PLAN objectives.
The HBO fluorescence illuminator provides an outstanding flexibility of use, standing the blue and green filter sets (supplied as standard) for Auramine, FITC, GFP and YFP (with blue filter set) plus Rhodamine, Texas Red and TRITC (with the green one), yet giving the possibility to combine any other specific filter sets for future upgrade. Transmitted light through the exclusive X-LED3 to ensure great-looking, rich and high-quality specimen view.

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B-510LD - LED Fluorescence Microscope

B-510LD_models

Model:  B-510LD1


Advanced routine fluorescence microscope for transmitted brightfield and fluorescence observations with IOS W-PLAN objectives.
The extremely powerful LED fluorescence illuminator is combined with blue excitation filter set for the visualization of the following fluorochromes: Acridine Yellow, Acridine Orange, Auramine, DiO, DTAF, FITC, GFP, YFP, etc. LED fluorescence ensures unparalleled convenience eliminating warm-up/cool-down times and all the inconveniences related lamp replacement and adjustment. Transmitted light through the exclusive X-LED3 to ensure great-looking, rich and high-quality specimen view.

B-510LD_models

Model:  B-510LD2


Advanced routine fluorescence microscope for transmitted brightfield and fluorescence observations with IOS W-PLAN objectives.
The extremely powerful LED fluorescence illuminator is combined with blue and green excitation filter set for the visualization of the following fluorochromes: Acridine Yellow, Acridine Orange, Auramine, DiO, DTAF, FITC, GFP, YFP, etc. (blue) plus Rhodamine, Texas Red and TRITC (green). LED fluorescence ensures unparalleled convenience eliminating warm-up/cool-down times and all the inconveniences related lamp replacement and adjustment. Transmitted light through the exclusive X-LED3 to ensure great-looking, rich and high-quality specimen view.

B-510FL - HBO Fluorescence Microscope

B-510FL_models

Model:  B-510FL


Advanced routine laboratory microscope for brightfield and fluorescence observations with Semi-Apo IOS W-PLAN F objectives to enhance the visibility of the sample and increase the overall contrast. The HBO fluorescence illuminator provides an outstanding flexibility of use, standing the blue and green filter sets (supplied as standard) for Auramine, FITC, GFP and YFP (with blue filter set) plus Rhodamine, Texas Red and TRITC (with the green one), yet giving the possibility to combine any other specific filter sets for future upgrade. Transmitted light through the exclusive X-LED3 to ensure great-looking, rich and high-quality specimen view.

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B-1000FL-LED - LED Fluorescence Microscope

B-1000FL-LED_model

Model:  B-1000FL-LED


The modular OPTIKA B-1000 can stand a LED fluorescence attachment, helping you working in a comfortable way during extended periods of use and performing reliable, accurate and rapid diagnosis benefiting from modularity, which gives the chance to create customized configurations tailored on customer needs. Versatile, robust, durable and sturdy, B-1000 offers premium quality optics (including Semi-Apo objectives), the state-of-the-art, exclusive X-LED8 (8 W) illumination system, designed by OPTIKA and the Koehler diaphragm. B-1000 gives multiple options as manual or motorized configuration, with a variety of objectives, stages and condensers.

B-1000FL-HBO - HBO Fluorescence Microscope

B-1000FL-HBO

Model:  B-1000FL-HBO


The modular OPTIKA B-1000 can stand a HBO fluorescence attachment, helping you working in a comfortable way during extended periods of use and performing reliable, accurate and rapid diagnosis benefiting from modularity, which gives the chance to create customized configurations tailored on customer needs. Versatile, robust, durable and sturdy, B-1000 offers premium quality optics (including Semi-Apo objectives), the state-of-the-art, exclusive X-LED8 (8 W) illumination system, designed by OPTIKA and the Koehler diaphragm. B-1000 gives multiple options as manual or motorized configuration, with a variety of objectives, stages and condensers.

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IM-3LD - LED Fluorescence Microscope

IM-3LD_models

Model:  IM-3LD


Routine inverted fluorescence microscope for transmitted brightfield, phase contrast and fluorescence observations with IOS LWD W-PLAN PH objectives.
The LED fluorescence illuminator is combined with blue and green excitation filter set for the visualization of the following fluorochromes: Acridine Yellow, Acridine Orange, Auramine, DiO, DTAF, FITC, GFP, YFP, etc. (blue) plus Rhodamine, Texas Red and TRITC (green). LED fluorescence ensures unparalleled convenience eliminating warm-up/cool-down times and all the inconveniences related lamp replacement and adjustment. Transmitted light through the exclusive X-LED8 to ensure great-looking, rich and high-quality specimen view.

IM-3F - HBO Fluorescence Microscope

IM-3LD_models

Model:  IM-3F


Routine inverted fluorescence microscope for transmitted brightfield, phase contrast and fluorescence observations with IOS LWD W-PLAN objectives. The HBO fluorescence illuminator is combined with blue and green excitation filter set for the visualization of the following fluorochromes: Acridine Yellow, Acridine Orange, Auramine, DiO, DTAF, FITC, GFP, YFP, etc. (blue) plus Rhodamine, Texas Red and TRITC (green).
Transmitted light through the exclusive X-LED8 to ensure great-looking, rich and high-quality specimen view.

IM-3LD_models

Model:  IM-3FL4


Advanced inverted microscope for brightfield and fluorescence observations with Semi-Apo IOS LWD U-PLAN F objectives to enhance the visibility of the sample and increase the overall contrast. The HBO fluorescence illuminator provides an outstanding flexibility of use, standing the blue and green filter sets (supplied as standard) for Auramine, FITC, GFP and YFP (with blue filter set) plus Rhodamine, Texas Red and TRITC (with the green one), yet giving the possibility to combine any other specific filter sets for future upgrade. Transmitted light through the exclusive X-LED8 to ensure great-looking, rich and high-quality specimen view.

IM-3F - HBO Fluorescence Microscope

IM-5FLD_models

Model:  IM-5FLD


OPTIKA IM-5FLD is designed to be the ultimate inverted research fluorescence microscope, combining innovative technology with unparalleled comfort. You can achieve astonishing images thanks to the IM-5FLD superb optics, Köhler condenser and the pioneering motorized selection of the more suitable LED source, automatically chosen in accordance with the selected fluorescence filter set (B, G, UV, plus optional ones). Last but not least, IM-5FLD boasts the highest F.O.V. available on an inverted microscope. Transmitted light through the exclusive X-LED8 to ensure great-looking, rich and high-quality specimen view.



Some detailed pictures and/or application examples showing the extreme versatility of B-510 Series in its several available configurations. This series represents the ideal solution for laboratories and customers looking for extreme reliability, comfort and accuracy during observations.